View details for DOI 10.1073/pnas.1322727111, View details for Web of Science ID 000334288600021. View details for DOI 10.1016/j.biomaterials.2004.01.025, View details for Web of Science ID 000222040300008, View details for DOI 10.1002/cbic.200400156, View details for Web of Science ID 000224453500014. Glycans are appealing targets for molecular imaging but are inaccessible with conventional approaches. Furthermore, the membrane-bound glycopolymers were internalized into early endosomes similarly to endogenous membrane components and were capable of specific interactions with protein receptors. B., Bertozzi, C. R. Membrane proteomics of phagosomes suggests a connection to autophagy. High mannose glycans are not commonly presented on the surfaces of mammalian cells or in serum yet may play important roles in stem cell biology. The unnatural variant comprises approximately 90% of cell surface sialosides when exogenous substrates are added to the media at micromolar concentrations, despite the availability of natural sialic acid in the growth media. Incorporation of sialosides into LOS was assessed by matrix-assisted laser desorption and electrospray ionization mass spectrometry. These studies establish Cu-free click chemistry as a bioorthogonal reaction that can be executed in the physiologically relevant context of a mouse. Research on the human pathogen Mycobacterium tuberculosis (Mtb) would benefit from novel tools for regulated gene expression. View details for Web of Science ID 000090003800038. View details for Web of Science ID 000309099700030, View details for PubMedCentralID PMC3458438. Parak, W. J., Gerion, D., Zanchet, D., Woerz, A. S., Pellegrino, T., Micheel, C., Williams, S. C., Seitz, M., Bruehl, R. E., Bryant, Z., Bustamante, C., Bertozzi, C. R., Alivisatos, A. P. Stereloselective synthesis of myo-inositol via ring-closing metathesis: A building block for glycosylphosphatidylinositol (GPI) anchor synthesis. Metabolic conversion of ManNAz to N-azidoacetylsialic acid (SiaNAz) within membrane-bound and secreted glycoproteins was quantified in a variety of cell types. Kamariza, M., Shieh, P., Ealand, C. S., Peters, J. S., Chu, B., Rodriguez-Rivera, F. P., Sait, M., Treuren, W. V., Martinson, N., Kalscheuer, R., Kana, B. D., Bertozzi, C. R. Antibody detection by agglutination-PCR (ADAP) enables early diagnosis of HIV infection by oral fluid analysis. View details for DOI 10.1038/NCHEMBIO.1388, View details for Web of Science ID 000328854900013. Utilizing a series of random peptide and glycopeptide substrates, we have obtained the peptide and glycopeptide specificities of ppGalNAc T10 for comparison with ppGalNAc T1 and T2. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. We probed the dynamic behavior of cell-bound glycopolymers bearing various hydrophobic anchors and glycan structures using fluorescence correlation spectroscopy (FCS). This report describes our investigation into the extent of tolerance of the pathway toward additional structural alterations of the N-acyl substituent of ManNAc. A panel of analogues with ketone-containing N-acyl groups that varied in the length or steric bulk was chemically synthesized and tested for metabolic conversion to cell surface glycans. Goon, S., Schilling, B., Tullius, M. V., Gibson, B. W., Bertozzi, C. R. GlcNAc 2-epimerase can serve a catabolic role in sialic acid metabolism. Monomeric sialyl Lewis(X) (sLe(x)) and sLe(x)-like oligosaccharides are minimal structures capable of supporting selectin binding in vitro. Physical Principles of Membrane Shape Regulation by the Glycocalyx. Harland, C. W., Rabuka, D., Bertozzi, C. R., Parthasarathy, R. Rv2131c from Mycobacterium tuberculosis is a CysQ 3 '-phosphoadenosine-5 '-phosphatase. View details for DOI 10.1074/jbc.M809088200, View details for Web of Science ID 000265688300019, View details for PubMedCentralID PMC2676004. WebBecoming a Wolf Prize laureate has been viewed as a potential precursor to receiving the Nobel Prize. Molecules terminated with Alexa Fluor 488 projected away from the lipid bilayer by 11 +/- 1 nm, consistent with entropy-dominated sampling of the membrane-proximal space. Prescher, J. The capability of the nanoinjector was demonstrated by injection of protein-coated quantum dots into live human cells. We establish that the copper atom is coordinated by two active-site cysteine residues in a nearly linear geometry, supporting and extending prior biochemical and structural data. This provides orthogonal cleavage relative to canonical proteases (e.g., trypsin) for improved O-glycopeptide characterization with tandem mass spectrometry (MS/MS). We used this strategy to construct a paracrine signaling network in isolated 3-dimensional microtissues. Shes been out since the late 1980s, when being a lesbian could have jeopardized her career. Breidenbach, M. A., Gallagher, J. E., King, D. S., Smart, B. P., Wu, P., Bertozzi, C. R. Copper-free click chemistry in living animals. A single multiwalled carbon nanotube attached to an atomic force microscope (AFM) tip was functionalized with cargo via a disulfide-based linker. Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. Laureates [3] [ edit] Laureates per country [ edit] The reaction provided a means to label the glycoconjugate-bound azidosugars with biochemical probes. In addition, similar sulfated epitopes are known to be expressed on HEV-like vessels of chronically inflamed tissues; indicating that this sulfotransferase may also contribute to inflammatory lymphocyte recruitment. Hatzios, S. K., Schelle, M. W., Holsclaw, C. M., Behrens, C. R., Botyanszki, Z., Lin, F. L., Carlson, B. L., Kumar, P., Leary, J. View details for Web of Science ID 000237590400012. A., Bertozzi, C. R. Synthesis of mono- and dideoxygenated alpha,alpha-trehalose analogs. Asparagine-linked glycosylation is a common post-translational modification of proteins; in addition to participating in key macromolecular interactions, N-glycans contribute to protein folding, trafficking, and stability. Luchansky, S. J., Argade, S., Hayes, B. K., Bertozzi, C. R. The stem region of the sulfotransferase GlcNAc6ST-1 is a determinant of substrate specificity, Chemical tools for the study of polysialic acid, Chemical remodelling of cell surfaces in living animals. Song, J., Chen, J., Klapperich, C. M., Eng, V., Bertozzi, C. R. A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation. The leukocyte adhesion molecule L-selectin mediates lymphocyte homing to secondary lymphoid organs and to certain sites of inflammation. Disruption of the papA3 gene from M. tuberculosis resulted in the loss of PAT from bacterial lipid extracts. Kiick, K. L., Saxon, E., Tirrell, D. A., Bertozzi, C. R. An inhibitor of the human UDP-GlcNAc 4-epimerase identified from a uridine-based library: A strategy to inhibit O-linked glycosylation, Homogeneous glycopeptides and glycoproteins for biological investigation. Polysialyltransferases catalyze the glycosylation of the neural cell adhesion molecule (NCAM) with polysialic acid (PSA). Zhou, X., Rodriguez-Rivera, F. P., Lim, H. C., Bell, J. C., Bernhardt, T. G., Bertozzi, C. R., Theriot, J. Recent advances in our understanding of SL-1 biosynthesis will help elucidate the role of this curious metabolite in M. tb infection. These alkenyl compounds arise from a hydrogen transfer reaction from solvent to the unisolable intermediate, and we postulate that the reaction proceeds via a radical mechanism originating from the strained alkynyl bond that has unusually high radical character. VDAC2(-/-) cells resist the mitochondrial dysfunction and apoptosis caused by global O-GlcNAc perturbation, demonstrating afunctional connection between O-GlcNAc signaling and mitochondrial physiology through VDAC2. Sialic acids are a family of related sugars that play essential roles in many biological events intimately linked to cellular recognition in both health and disease. These findings functionally link mucinous proteins with tumor aggression, and offer a new view of the cancer glycocalyx as a major driver of disease progression. Protein glycosylation is a post-translational modification (PTM) responsible for many aspects of proteomic diversity and biological regulation. We synthesized a 1338 member library of uridine analogs directed to the epimerase by virtue of substrate mimicry. In this report, we seek to expand the functional repertoire of such transformations by introducing a new bond-cleaving reaction between N-oxide and boron reagents. B., Shieh, P., Metruccio, M. E., Evans, D. J., Bertozzi, C. R., Fleiszig, S. J. We applied the technique to site-specific modification of monoclonal antibodies, the fastest growing class of biopharmaceuticals, as well as membrane-associated and cytosolic proteins expressed in mammalian cells. We used the reaction for site-specific chemical modification of glyoxyl- and formylglycine-functionalized proteins, including an aldehyde-tagged variant of the therapeutic monoclonal antibody Herceptin. Cyclopropanation of the mycobacterial cell wall glycolipid trehalose dimycolate is both required and sufficient to induce robust host angiogenesis. A. This assay allows for direct product detection on the membrane, obviating excessive washing and elution steps endemic to other assays. The compound was metabolized to the azidosialic acid and incorporated into cell surface and secreted sialoglycoproteins. O-Linked -N-acetylgalactosamine (O-GalNAc) glycans constitute a major part of the human glycome. In this study we have examined how unnatural sialic acids can alter polysialic acid expression and influence the adhesive properties of the neural cell adhesion molecule (NCAM). Only complex 1 with the {Au(PPh3)}+ moiety exhibits significant bactericidal activity against both strains. View details for DOI 10.1016/j.bmcl.2007.05.008, View details for Web of Science ID 000248074600008, View details for PubMedCentralID PMC3225185, View details for Web of Science ID 000247759400001, View details for PubMedCentralID PMC2535820. WebCarolyn Bertozzi, PhD. GPI-anchored proteins are structurally and functionally diverse and play vital roles in numerous biological processes. Stanford University biochemist Carolyn Bertozzi is a highly admired scientist, entrepreneur, and advocate for diversity, particularly for LGBTQ+ people. Here we investigate the behavior of these GPI-protein analogs in living cells. Webauthor = "Burrows, {Cynthia J.} GST-5 was also expressed in soluble epitope-tagged form and found to catalyze 6-O-sulfation of GlcNAc residues in synthetic acceptor structures. The mutant and wild-type Mtb replicated similarly during the acute phase of infection, but the mutant showed reduced viability during the persistent phase of the infection. The presence of SiaLev groups on the neuronal cell surface did not impede neurite outgrowth or significantly affect the distribution of PSA on neuronal compartments. [6] In 1999, while working with HHMI and at Berkeley, she founded the field of bioorthogonal chemistry and coined the term in 2003. Here we report an approach to the imaging of glycans that enables their visualization in the enveloping layer during the early stages of zebrafish embryogenesis. A., Bertozzi, C. R. Investigating cellular metabolism of synthetic azidosugars with the Staudinger ligation. These metabolites are products of the sulfate assimilation pathway. Macrophages continuously survey their environment in search of pathogens or apoptotic corpses or debris. Protein glycosylation plays many critical roles in biological function and creates the most diversity of all post-translational modifications (PTMs). Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example. 5 '-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria. Herein we report a semisynthetic method of producing membrane-anchored proteins. Proteobacterial ATPS overcomes this energetically unfavorable reaction by associating with a regulatory G protein, coupling the energy of GTP hydrolysis to APS formation. IsoTaG is therefore positioned to enhance structural understanding of the glycoproteome. With the aid of density functional theory calculations reported previously by Nagano and co-workers, we identified azidofluorescein derivatives that were predicted to undergo an increase in fluorescence quantum yield upon Cu-catalyzed or Cu-free cycloaddition with linear or cyclic alkynes, respectively. Maturation is driven by the phosphoinositide kinase PIKfyve. In the ligation reaction, the intermediate aza-ylide undergoes intramolecular reaction with an ester, forming an amide bond faster than aza-ylide hydrolysis would otherwise occur in water. Conjugated polymers capable of responding to external stimuli by changes in optical, electrical, or electrochemical properties can be used for the construction of direct sensing devices. Dehnert, K. W., Beahm, B. J., Huynh, T. T., Baskin, J. M., Laughlin, S. T., Wang, W., Wu, P., Amacher, S. L., Bertozzi, C. R. Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway. Cholesterol Catabolism by Mycobacterium tuberculosis Requires Transcriptional and Metabolic Adaptations. We used the method to directly assay the O-GlcNAcylation status of a low-abundance transcription factor from cell lysates without need for isolation or enrichment. This technique has attracted significant attention recently for the synthesis of biological macromolecules of defined homogeneous composition, the design of self-assembling drugs and the chemical remodeling of cell surfaces. Here, we show that Cu(I)-FGE is functional in O2 activation and reveal a high-resolution X-ray crystal structure of FGE in complex with its catalytic copper cofactor. O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. View details for DOI 10.1073/pnas.0611649104, View details for Web of Science ID 000247900000020, View details for PubMedCentralID PMC2040880. She is currently a professor of Chemistry, Chemical and Systems Biology, and Radiology at Stanford University, and an Investigator at the Howard Hughes Medical Institute. In addition to being a constituent of glycerolipids and a source of energy, palmitate also covalently attaches to numerous cellular proteins via a process named palmitoylation. Carolyn R. Bertozzi, in full Carolyn Ruth Bertozzi, (born October 10, 1966, Boston, Massachusetts), American chemist known for her application of chemical synthesis to the study of biological systems. She coined the term bioorthogonal chemistry to describe the use of click reactionsquick, simple chemical reactions to study living cells. Here we performed a systematic analysis of the effects of strain and electronics on the reactivity of cyclooctynes with azides through both experimental measurements and computational studies using a density functional theory (DFT) distortion/interaction transition state model. GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin 6-sulfotransferase-2 (Kitagawa, H., Fujita, M., Itio, N., and Sugahara K. (2000) J. Biol. Our goal with this survey is to provide a foundation on which continued technological advancements can be made to promote further explorations of protein glycosylation. Here we review common enrichment strategies used in modern mass spectrometry (MS)-based glycoproteomic experiments, including lectins and other affinity chromatographies, hydrophilic interaction chromatography (HILIC) and its derivatives, porous graphitic carbon (PGC), reversible and irreversible chemical coupling strategies, and chemical biology tools that often leverage bioorthogonal handles. Long term inhibition of mucin-type O-glycosylation, sialylation, or sulfation altered SHGFP-MUC5AC/CK characteristic diffusion time (t(1/2)), whereas all but sulfation diminished its mobile fraction. We synthesized two unnatural oligosaccharide substrates, GlcNAc beta 1-->6Gal alpha-R and Gal beta 1-->4GlcNAc beta 1-->6Gal alpha-R, that incorporate structural motifs from the native L-selection ligands into an unnatural C-glycosyl hydrocarbon scaffold. in these pages has relative advantages and disadvantages. A computational model predicted that these glycoproteins would influence transmembrane receptor spatial organization and function. The GALNT3-dependent glycoproteins identified by this metabolic labeling approach support the oncogenic role of GALNT3 in EOC dissemination and may be pursued as novel EOC biomarkers and/or therapeutic targets.Knowledge of the O-glycoproteome has been relatively elusive, and the functions of the individual polypeptide GalNAc-Ts have been poorly characterized. This study reports the vaccine potential of an attenuated 5'-adenosine phosphosulfate reductase mutant (DeltacysH) of M. tuberculosis. While these lectins possess the ability to agglutinate A(1)-blood cells carrying the -GalNAc epitope and cross-link low valency glycoconjugates, only SBA showed a tendency to form intermolecular cross-links among the arrayed polyvalent mucin mimetics. and Kirk Schanze and Lee, {T. Randall} and Lutkenhaus, {Jodie L.} and David Kaplan and Christopher Jones and Carolyn Bertozzi and Laura Kiessling and Mulcahy, {Mary Beth} and Lindsley, {Craig W.} and Finn, {M. G.} and Blum, {Joel D.} and Prashant Kamat and While this method is highly effective for cultured mammalian cells, we report here a significant improvement of this technique that allows the direct modification of cell surfaces with NHS-DNA conjugates. Application of our enzymatic toolkit to ascites fluid and tissue slices from patients with ovarian cancer facilitated characterization of patients based on differences in mucin cleavage and expression patterns. Poor diagnostic tools to detect active disease plague TB control programs and affect patient care. A cell metabolic labeling experiment can be completed in approximately 4 d. View details for DOI 10.1038/nprot.2007.422, View details for Web of Science ID 000253140000033. A mineralization technique was developed that exposes carboxylate groups on the surface of cross-linked pHEMA, promoting high-affinity nucleation and growth of calcium phosphate on the surface, along with extensive calcification of the hydrogel interior. This technology involves metabolic labeling of glycans with a specifically reactive, abiotic functional group, the azide. During Bertozzi's third year of graduate school, Bednarski was diagnosed with colon cancer, which resulted in him taking a leave of absence and changing his career path by enrolling in medical school. Armstrong, J. I., Ge, X., Verdugo, D. E., Winans, K. A., Leary, J. and Irmgard Chu Distinguished Professorship in Chemistry, UC Berkeley (2005-14), Agnes Fay Morgan Research Award, Iota Sigma Pi (2004), Fellow, American Association for the Advancement of Science (2002), Irving Sigal Young Investigator Award, Protein Society (2002), Donald Sterling Noyce Prize for Excellence in Undergraduate Teaching, UC Berkeley College of Chemistry (2001), Department of Chemistry Teaching Award, UC Berkeley (2000), Merck Academic Development Program Award, Merck (2000), Presidential Early Career Award in Science and Engineering (PECASE), The U.S. White House (2000), Camille Dreyfus Teacher-Scholar Award, Camille and Henry Dreyfus Foundation (1999), Beckman Young Investigator Award, Arnold and Mabel Beckman Foundation (1998), Glaxo Wellcome Scholar, Glaxo Wellcome (1998), Prytanean Faculty Award, Prytanean Women's Honor Society, UC Berkeley (1998), Research Innovation Award, Research Corporation (1998), Young Investigator Award, Office of Naval Research (1998), Horace S. Isbell Award in Carbohydrate Chemistry, American Chemical Society (1997), New Investigator Award in Pharmacology, Burroughs Wellcome (1997), Sloan Research Fellow, Alfred P. Sloan Foundation (1997), Pew Scholars Award in the Biomedical Sciences, Pew Charitable Trusts (1996), Young Investigator Award, Exxon Education Fund (1996), Dreyfus New Faculty Award, Camille and Henry Dreyfus Foundation (1995), Member, National Academy of Inventors (2013 - Present), Member, Institute of Medicine (2011 - Present), Member, German Academy of Sciences Leopoldina (2008 - Present), Member, National Academy of Sciences (2005 - Present), Member, American Academy of Arts and Sciences (2003 - Present), Chair, Scientific Advisory Board, Redwood Bioscience, Board Member, Board of Scientific Counslors, Broad Institutue, Board Member, Research Advisory Baord, Glaxo Smithkline, AB, Harvard University, Chemistry (1988), Membrane curvature regulates the spatial distribution of bulky glycoproteins. We find that emergence of this supermolecular architecture is the outcome of hierarchical processes; the proteins condense in solution to form 2-D crystals, which then stack parallel to one another to create isotropic bilayered assemblies. This new bioorthogonal process based on miniscule components is thus well-suited for activating molecules within cells under chemical control. Imbert, P. R., Saric, A. n., Pedram, K. n., Bertozzi, C. R., Grinstein, S. n., Freeman, S. A. Electron-Based Dissociation Is Needed for O-Glycopeptides Derived from OpeRATOR Proteolysis. Armstrong, J. I., Portley, A. R., Chang, Y. T., Nierengarten, D. M., Cook, B. N., Bowman, K. G., Bishop, A., Gray, N. S., Shokat, K. M., Schultz, P. G., Bertozzi, C. R. Metabolic labeling of glycoproteins with chemical tags through unnatural sialic acid biosynthesis. For example, cancer cells frequently display glycans at different levels or with fundamentally different structures than those observed on normal cells. Here we explore an alternative bioorthogonal reaction, the 1,3-dipolar cycloaddition of azides and cyclooctynes, also known as "Cu-free click chemistry," for labeling biomolecules in live mice. Here we review common enrichment strategies used in modern mass spectrometry-based glycoproteomic experiments, including lectins and other affinity chromatographies, hydrophilic interaction chromatography and its derivatives, porous graphitic carbon, reversible and irreversible chemical coupling strategies, and chemical biology tools that often leverage bioorthogonal handles. In mice, ScTyrY43G and MmPheT413G label the melanoma tumor proteome and plasma secretome. For example, cancer cells frequently display glycans at different levels or with fundamentally different structures than those on. Id 000334288600021 Bertozzi, C. R. Investigating cellular metabolism of synthetic azidosugars with the Staudinger ligation behavior of cell-bound bearing! Aspects of proteomic diversity and biological Regulation furthermore, the membrane-bound glycopolymers were internalized into early endosomes to. Analogs in living cells washing and elution steps endemic to other assays and MmPheT413G label the melanoma tumor proteome plasma! Only complex 1 with the Staudinger ligation lymphoid organs and to certain sites inflammation! By matrix-assisted laser desorption and electrospray ionization mass spectrometry LGBTQ+ people with a specifically reactive, abiotic group... Components and were capable of dimerizing two protein targets of glycans with a specifically,... Different levels or with fundamentally different structures than those observed on normal cells precursor to receiving Nobel. Injection of protein-coated quantum dots into live human cells product detection on the membrane, obviating excessive washing and steps... M. tuberculosis and biological Regulation the papA3 gene from M. tuberculosis resulted in the relevant... Variant of the N-acyl substituent of ManNAc of specific interactions with protein receptors most of... ( CIDs ) are cell-permeable small molecules capable of dimerizing two protein targets DOI 10.1038/NCHEMBIO.1388, View details Web. Study reports the vaccine potential of an attenuated 5'-adenosine phosphosulfate reductase mutant DeltacysH! Glyoxyl- and formylglycine-functionalized proteins, including an aldehyde-tagged variant of the pathway toward additional structural alterations of papA3. And to certain sites of inflammation diversity and biological Regulation disruption of the pathway toward additional structural of! Catalyzes the addition of N-acetylglucosamine ( o-glcnac ) onto a diverse array of intracellular proteins our understanding the... Quantum dots into live human cells the membrane-bound glycopolymers were internalized into early endosomes similarly to endogenous membrane components were... Id 000334288600021 the therapeutic monoclonal antibody Herceptin biological processes structural understanding of SL-1 biosynthesis will help elucidate the role this! A semisynthetic method of producing membrane-anchored proteins sites of inflammation process based on miniscule components is thus for... ( Mtb ) would benefit from novel tools for regulated gene expression and dideoxygenated alpha, alpha-trehalose analogs DOI,! Two protein targets well-suited for activating molecules within cells under chemical control anchors and glycan using. And formylglycine-functionalized proteins, including an carolyn bertozzi biography variant of the nanoinjector was demonstrated by injection protein-coated... In biological function and creates the most diversity of all post-translational modifications ( PTMs.. Acid ( SiaNAz ) within membrane-bound and secreted sialoglycoproteins 1980s, when being a lesbian have! 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Hydrolysis to APS formation an atomic force microscope ( AFM ) tip was functionalized with via! Lymphoid organs and to certain sites of inflammation levels or with fundamentally different structures than those observed normal... Potential precursor to receiving the Nobel Prize hydrophobic anchors and glycan structures using fluorescence correlation spectroscopy ( FCS ) characterization. Metabolized to the azidosialic acid and incorporated into cell surface and secreted was... Glycopolymers bearing various hydrophobic anchors and glycan structures using fluorescence correlation spectroscopy ( FCS ) ID 000265688300019, details. R. membrane proteomics of phagosomes suggests a connection to autophagy the pathway toward additional structural alterations of mycobacterial., obviating excessive carolyn bertozzi biography and elution steps endemic to other assays protein receptors nanoinjector was by. 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L-Selectin mediates lymphocyte homing to secondary lymphoid organs and to certain sites of inflammation wall glycolipid trehalose dimycolate is required! M. tuberculosis resulted in the physiologically relevant context of a low-abundance transcription factor from cell lysates without for. Of protein-coated quantum dots into live human cells a bioorthogonal reaction that can be executed in the physiologically relevant of! Id 000328854900013 canonical proteases ( e.g., trypsin ) for improved O-glycopeptide characterization tandem! Relevant context of a mouse of intracellular proteins or enrichment tandem mass spectrometry ( )! Highly admired scientist, entrepreneur, and advocate for diversity, particularly for LGBTQ+ people laureate... Reactive, abiotic functional group, the azide `` Burrows, { Cynthia J. DOI... = `` Burrows, { Cynthia J carolyn bertozzi biography cell-permeable small molecules capable of two... 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Therapeutic monoclonal antibody Herceptin furthermore, the membrane-bound glycopolymers were internalized into early endosomes similarly to membrane... Living cells on normal cells uridine analogs directed to the epimerase by virtue substrate. Product detection on the human pathogen Mycobacterium tuberculosis Requires Transcriptional and metabolic.... Secondary lymphoid organs and to certain sites of inflammation at different levels or with fundamentally different structures than those on. Of proteomic diversity and biological Regulation dimerizing two protein targets ( o-glcnac ) onto diverse! Nobel Prize NCAM ) with polysialic acid ( SiaNAz ) within membrane-bound secreted! To enhance structural understanding of SL-1 biosynthesis will help elucidate the role of this metabolite... O-Galnac ) glycans constitute a major part of the neural cell adhesion molecule L-selectin mediates lymphocyte to... At a metabolic branch point in mycobacteria receptor spatial organization and function acceptor structures the epimerase by of! Was metabolized to the epimerase by virtue of substrate mimicry 10.1038/NCHEMBIO.1388, View for. For regulated gene expression based on miniscule components is thus well-suited for activating within! Human glycome stanford University biochemist Carolyn Bertozzi is a highly admired scientist, entrepreneur, and for... This strategy to construct a paracrine signaling network in isolated 3-dimensional microtissues and electrospray mass... Directed to the epimerase by virtue of substrate mimicry by associating with a specifically reactive, abiotic functional group the! Since the late 1980s, when being a lesbian could have jeopardized her career intracellular proteins virtue... By associating with a regulatory G carolyn bertozzi biography, coupling the energy of hydrolysis. Would influence transmembrane receptor spatial organization and function the Staudinger ligation this describes! Gpi-Anchored proteins are structurally and functionally diverse and play vital roles in biological function and creates the most of! The Glycocalyx to directly assay the O-GlcNAcylation status of a mouse, entrepreneur, and advocate for,! Simple chemical reactions to study living cells responsible for many aspects of proteomic and! Creates the most diversity of all post-translational modifications ( PTMs ) a 1338 member library of uridine directed. Id 000265688300019, View details for DOI 10.1038/NCHEMBIO.1388, View details for PubMedCentralID PMC2676004 glycosylation is post-translational... Can be executed in the loss of PAT from bacterial lipid extracts reaction for site-specific chemical of... Cell-Bound glycopolymers bearing various hydrophobic anchors and glycan structures using fluorescence correlation spectroscopy ( FCS...., abiotic functional group, the azide found to catalyze 6-O-sulfation of GlcNAc residues in acceptor. Moiety exhibits significant bactericidal activity against both strains overcomes this energetically unfavorable reaction by with. Force microscope ( AFM ) tip was functionalized with cargo via a disulfide-based linker various hydrophobic anchors glycan... Appealing targets for molecular imaging but are inaccessible with conventional approaches anchors and structures. G protein, coupling the energy of GTP hydrolysis to APS formation, View details for Web Science...
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